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. 2017 May 14;9(5):497. doi: 10.3390/nu9050497

Figure 5.

Figure 5

EGCG ameliorates PA-BSA induced autophagic flux impairment in HK-2 cells. Western blot analysis of LC3-II, Beclin-1 and p62 in HK-2 cells pretreated with 40 μM EGCG for 24 h and subsequently incubated with PA-BSA (150 μM) for 12 h. (A) Western blot analysis of LC3-II, Beclin-1 and p62; (B) The relative protein levels of LC3-II; (C) Beclin-1 and (D) p62. β-Actin served as loading controls. Data are expressed as mean ± SEM (n = 3–5).* p < 0.05 vs. control group (BSA); # p < 0.05 vs. PA-BSA group.