Fig. 3. No-wash fixed cell confocal imaging of actin cytoskeleton. HeLa cells were incubated with phalloidin-TCO (1 μg mL^–1), washed and treated with 10 μM OG-5-Tz (a), Rh-5-Tz (b) or MeSiRh-5-Tz (c). Hoechst 33342 was used as nuclear stain. Images were taken 30 min post addition of the dyes without previous dye-washout.