Figure 6.
Genetic interaction tests indicate that CanA may function in a pathway that involves Ca2+ signaling, PKA, and PP1 function for refinement. A, The ectopic frequency observed in cacNT27/+ heterozygotes was reduced in genetic backgrounds heterozygous for CanA mutations (n = 54, 21, 29, 13, and 29, respectively; p = 0.003, 0.004, and 0.002, respectively). B, The elevated ectopic frequency observed in CanA14F/+ heterozygotes was reduced in mutant backgrounds that affect PKA and PP1 function (n = 20, 8, 12, 23, and 21, respectively; p = 0.02 and 0.04, respectively). C, A genetic background for increased PKA function suppresses the elevated ectopic frequency observed in cacNT27/+ heterozygotes and in larvae overexpressing PP1 pan-neurally (n = 12, 54, 19, 20, 10, 18, 12, 18, 21, and 22, respectively; p = 9.9 × 10−6, 0.04, 0.0004, and 8.2 × 10−6). *p < 0.05. **p < 0.01. ***p < 0.001.