Fig. 2.

ApoE isoforms differentially regulate the secretion of mature-BDNF. Primary human astrocytes were treated with cholesterol (Chol; 100 μM), with or without ApoE2 (20 nM) or ApoE3 (20 nM) or ApoE4 (20 nM) for 4 and 24 h. a and d Immunoblot showing expression levels of BDNF in the astrocyte-secreted media after 4 and 24 h respectively. ‘M’ indicates molecular weight marker. b and e ApoE2 + cholesterol induce the secretion of m-BDNF. c and f Expression levels of secreted pro-BDNF in different ApoE isoforms treated cells. Data are represented as mean ± SE of three independent experiments. (*P < 0.05, **P < 0.005 ***P < 0.0005 and ****P < 0.00005; ANOVA, post hoc Newman-Keuls multiple comparison)