Table 1.

DUSP5 PD(WT) inhibitor IC₅₀ values with time

	Assay Reaction Period
Inhibitor Compounds	0 – 10 min	15 – 25 min	30 – 40 min
MP Biomedicals	32.5 ± 2.8	5.9 ± 0.2	3.3 ± 0.2
RR535	86.6 ± 8.0	16.5 ± 0.7	6.3 ± 0.2
RR601	36.9 ± 3.9	36.5 ± 1.5	34.5 ± 1.4

IC₅₀ values (± SE) for MP Biomedicals, RR535, and RR601 were determined in the presence of DUSP5 PD(WT) as described in Fig. 4. Data was collected during three successive 10 min assays with pNPP as the substrate