Table 1.
mRNA panel used to analyse cell mRNA transcripts
| Gene Symbol | Gene Name | ENSEMBL Gene ID | Amplicon length | Assay number |
|---|---|---|---|---|
| Epithelial transcripts | ||||
| KRT8 | Keratin 8 | ENSG00000170421 | 164 | Hs01595539_g1 |
| KRT19 | Keratin 19 | ENSG00000171345 | 96 | AI70M8O (Custom assay) |
| EPCAM | Epithelial cell adhesion molecule | ENSG00000119888 | 64 | Hs00158980_m1 |
| CDH1 | Cadherin 1; type 1, E-cadherin | ENSG00000039068 | 65 | Hs01013953_m1 |
| EMT-associated transcripts | ||||
| VIM | Vimentin | ENSG00000026025 | 73 | Hs00185584_m1 |
| CDH2 | Cadherin 2; type 1, N-cadherin | ENSG00000170558 | 66 | Hs00983056_m1 |
| ZEB1 | Zinc finger E-box binding homeobox 1 | ENSG00000148516 | 63 | Hs00232783_m1 |
| Cancer stem cell transcripts | ||||
| CD24 | CD24 molecule | ENSG00000272398 | 140 | Hs02379687_s1 |
| CD44 | CD44 molecule | ENSG00000026508 | 70 | Hs01075861_m1 |
| ALDH1A1 | Aldehyde dehydrogenase 1; family member A1 | ENSG00000165092 | 61 | Hs00946916_m1 |
| Pancreas cancer-associated transcript | ||||
| SPARC | Secreted protein, acidic, cysteine-rich (osteonectin) | ENSG00000113140 | 76 | Hs00234160_m1 |
| Reference transcript | ||||
| HPRT1 | Hypoxanthine phosphoribosyltransferase 1 | ENSG00000165704 | 82 | Hs02800695_m1 |
| Leucocyte transcript | ||||
| PTPRC | Protein tyrosine phosphatase, receptor type C (CD45) | ENSG00000081237 | 57 | Hs04189704_m1 |