Table 2.
Primer list used in this study
| Oligonucleotide | Primer sequence (5′–3′) | Purpose |
|---|---|---|
| Homo arm-aprR cassette | ||
| F | TACCCGGCGGAGGCGTACCACCAGCAGTATCTGGACAAGAAATTCCGGGGATCCGTCGACC | Construction of pMSCPik311 |
| R | AGCGGCGCAGCCTCATGTAACGGGCCTGAGAGTGGCAGAAGCTTGCATTGTAGGCTGGAGCTGCCTC | |
| AprR cassette check | ||
| F | TACGGCGAGATCACCACGAC | Check for insertion of apr R cassette |
| R | GCGAGGATCGAGACGCGTGA | |
| PikD-kan | ||
| F | ACAACACCAAGCTTGCCCGCCT | Construction of pSAPDK |
| R | ACTGCAAGCTAGGCCCACCGATGAAGGACA | |
| F | TCGGTGGGCCTTAGCTTGCAGTGGGCTTACA | |
| R | TTGGTCGGTGAATTCGAACCCCAGA | |
| PikD-kan check | ||
| F | AGGCCCTGTTCGACGACCGA | Confirmation of pPik001 |
| R | TGCCTCGTCCTGCAGTTCATTCA | |
| PikRI-AI | ||
| F | CCCCTTCGAATTTCCGCCGC | Confirmation of pPik001 |
| R | CCGCCAGGAGTTCCCAGAAC | |
| PikAII | ||
| F | ACCGGTCTCAACTTCCGCGA | Confirmation of pPik001 |
| R | TCCAGGAGGGACAGGGTCAGC | |
| PikD | ||
| F | TCGCGGCACGGCATGGAGTACATGCA | Confirmation of pPik001 |
| R | TCAGGCCGTGACGGACTTGTCCT | |
| PikAI RNA | ||
| F | GACTGGGACCGCTTCTACCT | qRT-PCR |
| R | TACGAGACCGAGGAGGATCT | |
| DesIII RNA | ||
| F | GCTCTTTCTGGACCGCATAG | qRT-PCR |
| R | CGGTCATTTCGAAGCAGATT | |
| DesVI RNA | ||
| F | CATCTGGAGCACTTCACCAA | qRT-PCR |
| R | CTCGGTCGTCTTCAGGTAGC | |
| PikC RNA | ||
| F | GCTATCTCTCCCGGCTCATC | qRT-PCR |
| R | GATTGACCGTGGTCTCGTG | |
| PikD RNA | ||
| F | GGACGCTCTTACTCGTCTCC | qRT-PCR |
| R | CGGAGTAACTGGCAGAGGAC | |
| HrdB RNA | ||
| F | GCGGTGGAGAAGTTCGACTA | qRT-PCR |
| R | CACATGGTCGAGGTCATCAA | |