Fig. 1.

BPSL2105 organization and expression. (a) Predicted operon of B. pseudomallei BPSL2105/2106. (b) Demonstration of co-transcription of BPSL2105 and BPSL2106 by RT-PCR. RNA samples were collected from three separate cultures (1, 2 and 3). Primers used during RT-PCR are represented by the black arrows. (c) Expression of BPSL2105 during extreme heat shock. RNA samples were collected at 0, 10, 15 and 30 min time points, purified and residual DNA removed. Following this, the samples were amplified using RT-PCR with primers amplifying a region either from BPSL2105 or from 16S rRNA genes. Controls were carried out using RNA in the absence of reverse transcriptase. gDNA, genomic DNA.