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. 2015 Nov 1;161(Pt 11):2192–2203. doi: 10.1099/mic.0.000175

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© 2015 The Authors

Content includes material subject to Crown copyright (2015), Dstl. This material is licensed under the terms of the Open Government Licence except where otherwise stated. To view this licence, visit http://www.nationalarchives.gov.uk/doc/open-government-licence/version/3.

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Fig. 4. — Determining the oligomeric state of purified His₆-PspA protein. (a) Size exclusion chromatography trace of His₆-PspA showing a void volume at 49.5 ml containing the aggregated and higher-order PspA protein species, and a collection of peaks between 83.0 and 109.0 ml containing putative dimeric and monomeric His₆-PspA species. Included is an overlay of His₆-PspA protein purified under denaturing conditions. AU, absorbance units. (b) SDS-PAGE of His₆-PspA purified by gel filtration. Lanes represent pooled fractions from 0–10 (lane 1), 10–20 (lane 2), 20–30 (lane 3), 30–40 (lane 4), 40–50 (lane 5), 50–65 (lane 6), 65–80 (lane 7), 80–90 (lane 8), 90–100 (lane 9), 100–110 (lane 10) and 110–120 ml (lane 11). Values in kDa. (c) Western blot confirming the presence of His₆-PspA protein. Fractions are the same as those outlined in (b);+, His₆-PspA positive control.