| E. coli |
| BL21(DE3) |
F− ompT hsdSB(rB− , mB− ) gal dcm BL21(DE3) |
Invitrogen |
| DH5α |
F− ϕ80lacZΔM15 Δ(lacZYA–argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 |
E. coli genetic stock centre |
| S17-λ pir
|
λ pir recA thi pro hsdR −
RP4-2(Km : : Tn7 Tc : : Mu) TprSmr
|
Laboratory collection |
| B. pseudomallei |
| K96243 |
Clinical isolate from Thailand, sequenced strain |
Holden et al. (2004) |
| ΔpspA
|
K96243 derivative; unmarked deletion ΔBPSL2105
|
This study |
| ΔpspA/pBHR4.pspA
|
K96243 derivative; unmarked deletion ΔBPSL2105; pBHR4 : : BPSL2105, Cmr
|
This study |
| Plasmids |
| pCR-Blunt II-TOPO |
Cloning vector for blunt-end PCR products, Kmr
|
Invitrogen |
| pDM4 |
pNQ705 derivative, oriR6K, mobRP4, sacBR, Cmr
|
Milton et al. (1996) |
| pDM4.ΔpspA
|
pDM4 derivative with BPSL2105 flanking regions for mutagenesis, Cmr
|
This study |
| pBHR4-groS-RFP |
pBHR1 derivative, turboFP635, PgroES, rrnB, Cmr
|
Wand et al. (2011) |
| pBHR4.pspA
|
pBHR4-groS-RFP derivative with PBPSL2105, BPSL2105, Cmr
|
This study |
| pET28a |
E. coli expression vector, T7 lac promoter, Kmr
|
Novagen |
| pET28-His6-pspA
|
pET28a derivative, N-terminal His6-tagged BPSL2105, Kmr
|
This study |
