Table 2. Primers used.
| Primer | Sequence (5′ → 3′) | Restriction site |
|---|---|---|
| BPSL2105 LF F | AGATCTTGAACGCGTGCATGGAATCG | BglII |
| BPSL2105 LF R | CATATGTTTGATCGTGCGCGAAATAG | NdeI |
| BPSL2105 RF F | CATATGGACCGCCTCGAAGCGCTGAA | NdeI |
| BPSL2105 RF R | AGATCTCGAGCATGCCGCCCGAGGTC | BglII |
| BPSL2105 prom F | CGATCGGCGCTGAACGCGTGCATGGA | PvuI |
| BPSL2105 comp R | GGATCCTTACTGCGCCGGCGTGTTCA | BamHI |
| RT PspA F | CGCGCACGATCAAAGGTCTG | – |
| RT PspA R | GCCGCTGTTCGTATCGGCTG | – |
| 16S rRNA F | GATGACGGTACCGGAAGAATAAGC | – |
| 16S rRNA R | CCATGTCAAGGGTAGGTAAGGTTT | – |
| PspA F | GCCGCCGGATCCATGTCGCTTTTCGACTCTATTTC | BamHI |
| PspA R | CGGCGGGAGCTCTTACTGCGCCGGCGTGTTCAG | SacI |
| BPSL2105-6 F | GTCGGTCGACAAGCTCAAAG | – |
| BPSL2105-6 R | GGATCGGTGAAGCGCAGTTG | – |
Restriction sites are underlined.