Table 1.
The nTDMS fragmentation propensities ordered from the most frequently cleaved residue pairs (top) to the least frequently cleaved residues pairs (bottom). Residue pairs with fragmentation propensities >20% and number of assigned fragmentation events >13 are shown above the gray bar. In contrast, residue pairs with lower than expected fragmentation propensities (<5%) and >275 possible fragmentation events are shown below the gray bar.
| Residue pair | Residue N-terminal to fragmentation | Residue C-terminal to fragmentation | Assigned fragmentation events | Possible fragmentation events | Fragmentation propensity |
|---|---|---|---|---|---|
| D|P | Aspartic Acid | Proline | 121 | 190 | 64% |
| D|C | Aspartic Acid | Cysteine | 32 | 66 | 49% |
| D|G | Aspartic Acid | Glycine | 171 | 354 | 48% |
| D|Y | Aspartic Acid | Tyrosine | 59 | 126 | 47% |
| F|W | Phenylalanine | Tryptophan | 14 | 30 | 47% |
| D|D | Aspartic Acid | Aspartic Acid | 100 | 220 | 46% |
| D|M | Aspartic Acid | Methionine | 37 | 84 | 44% |
| D|K | Aspartic Acid | Lysine | 124 | 304 | 41% |
| D|Q | Aspartic Acid | Glutamine | 21 | 52 | 40% |
| D|S | Aspartic Acid | Serine | 56 | 144 | 39% |
| D|V | Aspartic Acid | Valine | 68 | 176 | 39% |
| E|P | Glutamic Acid | Proline | 46 | 120 | 38% |
| D|A | Aspartic Acid | Alanine | 85 | 224 | 38% |
| D|I | Aspartic Acid | Isoleucine | 71 | 194 | 37% |
| D|E | Aspartic Acid | Glutamic Acid | 95 | 260 | 37% |
| D|T | Aspartic Acid | Threonine | 34 | 96 | 35% |
| D|H | Aspartic Acid | Histidine | 21 | 60 | 35% |
| A|P | Alanine | Proline | 77 | 234 | 33% |
| D|N | Aspartic Acid | Asparagine | 38 | 116 | 33% |
| Y|P | Tyrosine | Proline | 26 | 80 | 33% |
| V|P | Valine | Proline | 60 | 192 | 31% |
| D|R | Aspartic Acid | Arginine | 48 | 156 | 31% |
| W|A | Tryptophan | Alanine | 17 | 58 | 29% |
| L|H | Leucine | Histidine | 21 | 72 | 29% |
| K|T | Lysine | Threonine | 75 | 282 | 27% |
| I|P | Isoleucine | Proline | 35 | 132 | 27% |
| L|P | Leucine | Proline | 56 | 212 | 26% |
| D|L | Aspartic Acid | Leucine | 83 | 336 | 25% |
| H|P | Histidine | Proline | 15 | 64 | 23% |
| N|P | Asparagine | Proline | 21 | 92 | 23% |
| Y|I | Tyrosine | Isoleucine | 17 | 76 | 22% |
| V|G | Valine | Glycine | 66 | 302 | 22% |
| E|G | Glutamic Acid | Glycine | 63 | 290 | 22% |
| Q|P | Glutamine | Proline | 18 | 84 | 21% |
| L|M | Leucine | Methionine | 21 | 98 | 21% |
| A|A | Alanine | Alanine | 18 | 392 | 5% |
| A|L | Alanine | Leucine | 16 | 358 | 5% |
| K|D | Lysine | Aspartic Acid | 16 | 388 | 4% |
| G|K | Glycine | Lysine | 20 | 498 | 4% |
| G|L | Glycine | Leucine | 9 | 332 | 3% |
| S|K | Serine | Lysine | 8 | 304 | 3% |