Table 1.
Sequence and efficiency of the primers used in this study
| Gene | NCBI reference sequence | P (bp) | E (%) | Forward primer | Reverse primer |
|---|---|---|---|---|---|
| bGapdh | NM_001034034.2 | 90 | 101 | CACCCACGGCAAGTTCAAC | TCTCGCTCCTGGAAGATGGT |
| bRps14 | NM_001077830.2 | 125 | 105 | CATCACTGCCCTCCACATCA | TTCCAATCCGCCCAATCTTCA |
| bAlk1 | NM_001083479.1 | 109 | 100 | ACAACACAGTGCTGCTCAGACA | TGCTCGTGGTAGTGCGTGAT |
| bAlk2 | NM_176663.3 | 61 | 105 | CGTTGGAGACAGCACTTTAGCA | AGAGCCGCTTCCCGATGTA |
| bAlk3 | NM_001076800.1 | 142 | 96 | GAGCAAGATGAAGCATTTATTCCA | CAACCTGCCGAACCATCTG |
| bAlk5 | NM_174621.2 | 75 | 94 | CAGGACCACTGCAATAAAATAGAACTT | TGCCAGTTCAACAGGACCAA |
| bSerpine1 | NM_174137.2 | 55 | 99 | CGAGCCAGGCGGACTTC | TGCGACACGTACAGAAACTCTTGA |
| bId1 | NM_001097568.2 | 73 | 107 | GCTCCGCTCAGCACTCTCAA | GATCGTCCGCTGGAACACA |
| bMmp3 | NM_001206637.1 | 130 | 106 | GAATCTGTGCCTCCCGAACC | TCCTGAAAGATTTCCGCCAA |
| bSmad7 | NM_001192865.1 | 72 | 98 | GGGCTTTCAGATTCCCAACTT | CTCCCAGTATGCCACCACG |
| bTgfb1 | NM_001166068.1 | 80 | 107 | GGTGGAATACGGCAACAAAATCT | GCTCGGACGTGTTGAAGAAC |
| bJunb | NM_001075656.1 | 139 | 97 | CCTTCTACCACGACGACTCA | CCGGGTGCTTTGAGATTTCG |
| bNgf | NM_001099362.1 | 110 | 85 | CAGCTCTTTTGATCGGCATACA | TGTGTCAAGGGAATGCTGAAGT |
| bFn1 | NM_001163778.1 | 95 | 96 | GCACCACTCCCGACATTACT | CTGATCGGCATGGACCACTT |
| bCol2a1 | NM_001001135.2 | 60 | 97 | TGATCGAGTACCGGTCACAGAA | CCATGGGTGCAATGTCAATG |
P is product length in base pairs, E is efficiency in percentage. Alk1 is also known as Acvrl1, Alk2 as Acvr1, Alk3 as Bmpr1a and Alk5 as Tgfbr1