Fig. 7.

Inhibition of P450 activities for oprozomib in human hepatocytes by 1-ABT. (A) Percentage of oprozomib remaining in human hepatocytes. (B) Formation of PR-176 in human hepatocytes. Cryopreserved human hepatocytes were incubated with oprozomib (2 μM) after preincubation with 1-ABT (0.5 mM) or vehicle (0.1% DMSO) for 30 minutes. Both oprozomib disappearance and PR-176 formation after various incubation times were quantified using LC-MS/MS. Data represent mean ± S.D. from triplicate incubations. The percentage of oprozomib was calculated using the remaining oprozomib concentration divided by zero-minute oprozomib concentration.