TABLE 4.
Primer | Sequence (5′→3′) | Referencea |
---|---|---|
Forward | ||
Vβ1 | GTTGATTCGAAATGAGACGGTGCCC | 25 |
Vβ2 | GGAGTCCTGGGGACAAAGAGGTCA | |
Vβ3 | GAGGTGTATCCCTGAAAAGG | 42 |
Vβ4 | CCTGATATGCGAACAGTATCTAGGC | 25 |
Vβ5 | CCCAGCAGATTCTCAGTCCAACAG | 25 |
Vβ6 | GCGATCTATCTGAAGGCTATGATGC | 25 |
Vβ7 | GTACTGGTATCGACAAGACCC | 42 |
Vβ8 | GCATGGGCTGAGGCTGATCCATTA | 25 |
Vβ9 | GAACAGGGAAGCTGACAC | 42 |
Vβ10 | TCCAAGGCGCTTCTCACCTCAGTC | 25 |
Vβ11 | TGCTGGTGTCATCCAAACACCTAG | 25 |
Vβ12 | AGTTACCCAGACACCCAGACATGA | 25 |
Vβ13 | CTGCTGTGAGGCCTAAAGGAACTA | 25 |
Vβ14 | AGAGTCGGTGGTGCAACTGAACCT | 25 |
Vβ15 | CCCATCAGTCATCCCAACTTATCC | 25 |
Vβ16 | TAGGACAGCAGATGGAGTTTCTGG | 25 |
Vβ17 | GCACACTGCCTTTTACTGG | 42 |
Vβ18 | GGACATCTGTCAAAGTGGC | 42 |
Vβ19 | CTACAAGAAACCGGGAGAAGAACTC | 26 |
Vβ20 | GCCAGGAAGCAGAGATG | 42 |
Dβ1 | GCTTATCTGGTGGTTTCTTCCAGC | 25 |
Dβ2 | GTAGGCACCTGTGGGGAAGAAACT | 25 |
β-actin F | GGCTGTATTCCCCTCCATCG | |
β-actin F nested | TGGGGTTTTCTTGGGGATCG | |
Reverse | ||
Jβ1 R | CAGAGAAGAGCAAGCGACCA | |
Jβ2 R | TGAGAGCTGTCTCCTACTATCGATT | 25 |
Jβ1 R nested | AAGGGACGACTCTGTCTTACCTTATACC | 82 |
Jβ2 R nested | TTTCCCTCCCGGAGATTCCCTAA | 25 |
β-actin R | CAATTGAGAAAGGGCGTGGC |
Primers without a reference indicated were designed for this study.