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. 2017 May 31;37(12):e00052-17. doi: 10.1128/MCB.00052-17

TABLE 4.

Primers used for analysis of Tcrb gene rearrangement

Primer Sequence (5′→3′) Referencea
Forward
    Vβ1 GTTGATTCGAAATGAGACGGTGCCC 25
    Vβ2 GGAGTCCTGGGGACAAAGAGGTCA
    Vβ3 GAGGTGTATCCCTGAAAAGG 42
    Vβ4 CCTGATATGCGAACAGTATCTAGGC 25
    Vβ5 CCCAGCAGATTCTCAGTCCAACAG 25
    Vβ6 GCGATCTATCTGAAGGCTATGATGC 25
    Vβ7 GTACTGGTATCGACAAGACCC 42
    Vβ8 GCATGGGCTGAGGCTGATCCATTA 25
    Vβ9 GAACAGGGAAGCTGACAC 42
    Vβ10 TCCAAGGCGCTTCTCACCTCAGTC 25
    Vβ11 TGCTGGTGTCATCCAAACACCTAG 25
    Vβ12 AGTTACCCAGACACCCAGACATGA 25
    Vβ13 CTGCTGTGAGGCCTAAAGGAACTA 25
    Vβ14 AGAGTCGGTGGTGCAACTGAACCT 25
    Vβ15 CCCATCAGTCATCCCAACTTATCC 25
    Vβ16 TAGGACAGCAGATGGAGTTTCTGG 25
    Vβ17 GCACACTGCCTTTTACTGG 42
    Vβ18 GGACATCTGTCAAAGTGGC 42
    Vβ19 CTACAAGAAACCGGGAGAAGAACTC 26
    Vβ20 GCCAGGAAGCAGAGATG 42
    Dβ1 GCTTATCTGGTGGTTTCTTCCAGC 25
    Dβ2 GTAGGCACCTGTGGGGAAGAAACT 25
    β-actin F GGCTGTATTCCCCTCCATCG
    β-actin F nested TGGGGTTTTCTTGGGGATCG
Reverse
    Jβ1 R CAGAGAAGAGCAAGCGACCA
    Jβ2 R TGAGAGCTGTCTCCTACTATCGATT 25
    Jβ1 R nested AAGGGACGACTCTGTCTTACCTTATACC 82
    Jβ2 R nested TTTCCCTCCCGGAGATTCCCTAA 25
    β-actin R CAATTGAGAAAGGGCGTGGC
a

Primers without a reference indicated were designed for this study.