Figure 10.

Theoretically and experimentally probing the effects of an allosteric regulator on activity. Data points show experimentally measured activity from Feller et al. for the enzyme α-amylase using substrate analogue [S] (EPS) and allosteric activator [R] (NaCl).⁵² Best fit theoretical curves described by eq 63 are overlaid on the data. The best fit parameters are e^{−β(ε_A−ε_I)} = 7.8 × 10⁻⁴, $K_{\mathrm{M}}^{\mathrm{A}}=0.6\text{mM},K_{\mathrm{M}}^{\mathrm{I}}=0.2\text{mM},R_{\mathrm{D}}^{\mathrm{A}}=0.03\text{mM},R_{\mathrm{D}}^{\mathrm{I}}=7.9\text{mM},k_{\text{cat}}^{\mathrm{A}}=14{\mathrm{s}}^{-1}$, and $k_{\text{cat}}^{\mathrm{I}}=0.01{\mathrm{s}}^{-1}$.