Figure 1.

Growth of odr1 and ysp2 overexpressor strains on solid media in presence and absence of thiamine. Cells containing the plasmids pODR1 and pYSP2 under the control of the nmt1 promoter, along with the control cells containing the plasmids pREP4X and wild-type cells 972, were spotted at two different cell densities on solid MM containing 2% and 0.5% glucose (Glc). Plates contain thiamine and 2-DG as indicated (Materials and Methods). Growth resulting after plating ∼1.6 × 10⁴ and 0.4 × 10⁴ cells as 5 μl drops was assayed. When cells were diluted more, they were not growing at all or only very poorly on 2-DG plates, indicating that 2-DG resistance is highly dependent on initial cell density.