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Growth inhibition of deletion mutants by 2-DG in liquid medium. The mutants as indicated, along with the parent strain (ade6-M210 ura4-D18 leu1-32 h+), were grown at 30°C in MM medium containing 2% glucose (A) or 0.5% glucose (B) in the presence and absence of 2-DG (0.25 mg/ml). Growth was tested after 2 and 4 h and percentages of growth inhibition by 2-DG were plotted. The data represent the mean of two independent experiments.
