Table 2.
Ratios of invertase activity of 2-DG-resistant mutants under glucose-repressed and derepressed conditions and in the presence of 2-DG.
| Strain | Fold increase of invertase activity from 2% to 0.5% glucose | Fold increase of invertase activity in 2% glucose containing 2-DG |
|---|---|---|
| pREP4X | 3.9 ± 0.26 | 2.2 ± 0.19 |
| pODR1 | 1.6 ± 0.06 | 1.3 ± 0.07 |
| Parent | 5.0 ± 0.44 | 1.7 ± 0.6 |
| odr1Δ | 6.9 ± 0.83 | 3.6 ± 0.61 |
| snf5Δ | 1.1 ± 0.01 | 1.2 ± 0.19 |
| ypa1Δ | 1.9 ± 0.22 | 1.1 ± 0.05 |
| pas1Δ | 1.2 ± 0.004 | 1.0 ± 0.03 |
| pho7Δ | 1.0 ± 0.08 | 1.0 ± 0.06 |
pREP4X: control strain for overexpressor strain pODR1.
Parent: parent strain of deletion strains (ade6-M210 ura4-D18 leu1-32 h+).
Different ratios of activities in pREP4X and parent strains are due to plasmid effects. Activity was measured in MM containing 2%, 0.5% or 2% glucose plus 0.25 mg/ml 2-DG (see Materials and Methods) and the ratios ±SEM (n = 3) are indicated here.