TABLE 3.

Integration events characterized by LAM-PCR and DNA sequencing^a

IE	Cell line	Relaxase	No. of sequences	Identity (%)	Chr	Integration locus	Missing bp
1	EA.hy926	TrwC	19	99.75	11	35225119	0
2	EA.hy926	TrwC	402	100	11	35225119	0
3	HeLa	TrwC::BID	15	98.25	2	37383046	15
4	HeLa	TrwC::BID	114	100	2	111118923	16
5	HeLa	TrwC::BID	15	100	6	9173423	18
6	HeLa	TrwC::BID	84	99.48	12	28128063	16
7	HeLa	TrwC::BID	21	100	13	83099211	0
8	HeLa	TrwC::BID	95	99.72	16	68832248	16
9	HeLa	TrwC::BID	15	100	19	18303607	14

^aThe information collected for each integration event is shown. Number of sequences indicates the number of times the sequence read was found. Missing base pairs indicates the number of bp that are missed in the read with respect to the plasmid sequence until the nic site. IE, integration event; Chr, chromosome.