TABLE 4.

Mapped illegitimate integration events^a

IS	Sequence (5′ to 3′)	Integration junction
		Chr	Genomic locus	Plasmid
2	AAAATGAGGACAGTT/ATATTTTTTAAATGT	11	35225119	nic+1
3	CCAGATCGTGCCACT/GCATTCCAGCCTGGC	2	37383046	nic−15
4	TGGGAAACAAATGAA/GAAACAACCCTGCTG	2	111118923	nic−16
5	GTTTCCATGGACATT/TGCCACCCCGGCTTC	6	9173423	nic−18
6	CGGGTTAGAAACCAA/GCACCCAAGCCGGCG	12	28128063	nic−16
8	CACTTGCTGGGCTCA/GAGACAACCCAGCCC	16	68832248	nic−16
9	GTTGTAACTGCCTAA/GATTGACCAACCCTA	19	18303607	nic−14
10	GTCACATGATAAAAA/GATTATTTCATTTTG	15	60623276	gfp_73
11	ATTTAATCCAAATAG/AAATAAGTTTCAGAT	15	60724330	trwA_353
oriT	AGGTGCGTATTGTCT/ATAGCCCAGATTTAA			nic

^aThe genomic integration sites are shown aligned with the wild-type target for TrwC, the oriT gene. The slash in each sequence indicates the integration site (the nic site in the oriT sequence). The location of the integration site (Chr, chromosome number), as well as the nucleotide of the plasmid by which integration took place, is also displayed. IS, integration site. IS2 to -9 were characterized by LAM-PCR. IS10 and -11 are both integration junctions of the event characterized by recovery of the integrated plasmid. Coordinates of genomic loci correspond to human genome GRCh37/hg19 available in UCSC Genome Browser. Plasmid coordinates refer to the distance from the nic site (+ and − indicating in the 5′or 3′ direction from the nic site, respectively) for IS2 to -9, or the nucleotide position in the gfp and trwA open reading frames (ORFs) for IS10 and -11.