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Knockdown of mecA in S. aureus N315 by CRISPRi. (A) Genetic organization of the mecA gene and the sgRNA binding site. (B) Oxacillin susceptibilities of knockdown (MSD1) and control (MSD0) strains detected by disk diffusion test. Daptomycin (a non-beta-lactam antibiotic) was used as a control. Abbreviations: OXA, oxacillin; DAP, daptomycin; MSD0, N315 carrying plasmid pSD1; MSD1, N315 expressing the mecA sgRNA1.
