TABLE 2.
Sequences of primers used in this study
| Primer | Sequence (5′–3′) |
|---|---|
| PRMC2-NcoI-F | CATTCTCTGGTATTTGGACTCCTG |
| PRMC2-SapI-R | AGTCAGTGAGCGAGGAAGCGTAAGAGCGCCCAATAC |
| PRMC2-SapI-F | GTATTGGGCGCTCTTACGCTTCCTCGCTCACTGACT |
| PRMC2-EcoRI-R | AGCTTGATGGTACCGTTAACAGA |
| PpflB-SmaI-F | TAGCCCCGGGATATACTCCTAAATTAACTT |
| PpflB-R | TAGTCATTGTAGCATGTTTGTTGTG |
| sgRNA-SapI-F | CACAACAAACATGCTACAATGACTATGAAGAGC CATGTCAGGCTCTTCTGT |
| sgRNA-SapI-R | ACTTGCTATTTCTAGCTCTAAAACAGAAGAGCCT GACATGGCTCTTCATAG |
| sgRNA-F | GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAA |
| sgRNA-R | AAAAAAGCACCGACTCGGTGCCACTTTTTCAAG TTGATAACGGACTAGCCTTATTT |
| sgRNA-slice-BglII-F | GAAGATCTTAGCCCCGGGATATACTCC |
| sgRNA-slice-EcoRI-R | CGGAATTCAAAAAAGCACCGACTCGG |
| sgRNA-cassette-F | CGGAATTCATATACTCCTAAATTAACTT |
| sgRNA-cassette-R | CGGAATTCAAAAAAGCACCGACTCGG |
| dcas9-BglII-F | GAAGATCTGGGAGGCCGTTTCATGGATAA |
| dcas9-SmaI-R | TCCCCCGGGTATAAACGCAGAAAGGCCCAC |
| hla-sgRNA1-oligo1 | CTAGTTGAATAAATTCTTTATGAAACA |
| hla-sgRNA1-oligo2 | AACTGTTTCATAAAGAATTTATTCAAC |
| hla-sgRNA2-oligo1 | CTACATTAGCGACAGGATTCATTAATA |
| hla-sgRNA2-oligo2 | AACTATTAATGAATCCTGTCGCTAATG |
| hla-sgRNA3-oligo1 | CTACGGGTTCCAAGAATCTCTATCATA |
| hla-sgRNA3-oligo2 | AACTATGATAGAGATTCTTGGAACCCG |
| hla-sgRNA4-oligo1 | CTACTCAGTAACAACAACACTATTGCT |
| hla-sgRNA4-oligo2 | AACAGCAATAGTGTTGTTGTTACTGAG |
| mecA-sgRNA1-oligo1 | CTAACAACTACAACTATTAAAATAAG |
| mecA-sgRNA1-oligo2 | AACCTTATTTTAATAGTTGTAGTTGT |
| spa-sgRNA1-oligo1 | CTATCGTGTTGCGCAGCATTTGCAGC |
| spa-sgRNA1-oligo2 | AACGCTGCAAATGCTGCGCAACACGA |
| sarT-sgRNA1-oligo1 | CTATAACTCCAAATATATTTAGTTTG |
| sarT-sgRNA1-oligo2 | AACCAAACTAAATATATTTGGAGTTA |
| sarT-sgRNA2-oligo1 | CTATTACGCTTTTAACAATTCTAGAT |
| sarT-sgRNA2-oligo2 | AACATCTAGAATTGTTAAAAGCGTAA |
| ccrAB-sgRNA1-oligo1 | CTAATAGCCTATGACTTGTTTCATAT |
| ccrAB-sgRNA1-oligo2 | AACATATGAAACAAGTCATAGGCTAT |
| ccrAB-sgRNA2-oligo1 | CTAGATATGTTGAATATGATGTTTTT |
| ccrAB-sgRNA2-oligo2 | AACAAAAACATCATATTCAACATATC |
| ccrAB-sgRNA3-oligo1 | CTACGTTTATGCAATCGATGATTGCA |
| ccrAB-sgRNA3-oligo2 | AACTGCAATCATCGATTGCATAAACG |
| ccrAB-sgRNA4-oligo1 | CTAATATATCTTTAACTTCAAAATGA |
| ccrAB-sgRNA4-oligo2 | AACTCATTTTGAAGTTAAAGATATAT |
| era-sgRNA1-oligo1 | CTAATATCGTCTATTTGTATAATTCC |
| era-sgRNA1-oligo2 | AACGGAATTATACAAATAGACGATAT |
| era-sgRNA2-oligo1 | CTACCCTAAAATAACGCTATAAAATG |
| era-sgRNA2-oligo2 | AACCATTTTATAGCGTTATTTTAGGG |
| era-sgRNA3-oligo1 | CTAACAAATGTTGACTTTCCTACATT |
| era-sgRNA3-oligo2 | AACAATGTAGGAAAGTCAACATTTGT |
| era-sgRNA4-oligo1 | CTACTGCTTTGATGATAATCCCTTTT |
| era-sgRNA4-oligo2 | AACAAAAGGGATTATCATCAAAGCAG |
| murE-sgRNA-oligo1 | CTAATACTAGCTACTCTTAATGTGTC |
| murE-sgRNA-oligo2 | AACGACACATTAAGAGTAGCTAGTAT |
| rpsC-sgRNA1oligo1 | CTAATAATACCAACACGAAGTCCGAT |
| rpsC-sgRNA-oligo2 | AACATCGGACTTCGTGTTGGTATTAT |
| walR-sgRNA-oligo1 | CTATTAAATTCTAAAATATCAGCAAT |
| walR-sgRNA-oligo2 | AACATTGCTGATATTTTAGAATTTAA |
| RT-pta-F | AAAGCGCCAGGTGCTAAATTAC |
| RT-pta-R | CTGGACCAACTGCATCATATCC |
| RT-hla-F | AAAGTAGGCTGGAAAGTGAT |
| RT-hla-R | TAGCGAAGTCTGGTGAAAA |
| RT-spa-F | AAGATGGTAACGGAGTACATGTCG |
| RT-spa-R | CAAGTTCTTGACCAGGTTTGATC |
| RT-ccrA-F | GCACAGTTATTAGAAGAAGATA |
| RT-ccrA-R | GCCATATTGATTGTTGACA |
| RT-ccrB-F | CAATACCACGAATACACTT |
| RT-ccrB-R | CATCACATAATCTTCAATCAC |
| RT-era2-F | TGCTTTAGAAGAAGATGAGCCAGAG |
| RT-era2-R | GTCCGTAATTGTTTGCTTGTTCT |
| RT-era4-F | GTTAGAAAAGCACAACAAGAATC |
| RT-era4-R | TAGCTCGTTCAGCACATATCG |
| RT-era5-F | AAGGTGTTATGACAAGAGATGACG |
| RT-era5-R | AGCGACTTTCATCATATAGTCACC |
| RT-era6-F | GGTGCAAAAGTACCACTTATGGC |
| RT-era6-R | TTCCCATACCTCGCCACTGATT |
| pRMC2-seq-F | CACAGATGCGTAAGGAGA |