FIG 5.

In vivo mutualism phenotypes. Bacterial strains with in-genome constructs expressing fixed levels of Lrp were coinjected into five Galleria insects per treatment. Insects that produced more than 50 emerged IJs were considered productive insects. (A) Percent productive insect is calculated as the percentage of productive insects out of the total number of insects infected per experiment. (B) Emerged and colonized IJs per productive insect were calculated as the colonization frequency multiplied by the number of emerged IJs per productive insect. Data are presented as means with standard errors (n = 3 biological replicates; each biological replicate is the average for five insects). One-way ANOVA was used to determine statistical significance (***, P ≤ 0.001).