TABLE 4.
Plasmids and primers used in this study
| Plasmid or primer | Description or sequence (5′ to 3′) | Reference or source |
|---|---|---|
| Plasmids | ||
| pRM4 | pBAD18 Cmr; gp57A (SacI/SalI) | 43 |
| pAVI-S16LTF | pETDUET Ampr; MCSI:AVI-LTF (BamHI/PstI); MCSII:BirA (NdeI/XhoI) | This study |
| pHIS-S16LTF | pETDUET Ampr backbone; MCSI:LTF (BamHI/NotI); MCSII:S16 gp57A chaperone (NdeI/XhoI) | This study |
| Primers | ||
| Avi1 | GATCCGGCGGGTGGCGGTCTGAACGACATCTTCGAGGCTCAGAAAATCGAATGGCACGAA | |
| Avi2 | TCGACTTCGTGCCATTCGATTTTCTGAGCCTCGAAGATGTCGTTCAGACCGCCACCCGCCG | |
| LTF1_SalI | ATTCGTCGACGAGCTCATGGCTACTATAAA | |
| LTF2_PstI | TTTTTTCTGCAGTTATAACCAAGAACCAGCAATATTACC | |
| LTF3_BamHI | GCCAGGATCCGGCGGGTGGC | |
| LTF4_NdeI | CGCCATATGACTGATAAAATTAAACAGCT | |
| LTF5_XhoI | CCGCTCGAGTCATTCATCATCCGGCGTT | |
| LTF6_BamHI | CGCGGATCCGGAGAATCTGTATTTCCAGGGAATGGCTACTATAAAACAAATACAA | |
| LTF7_NotI | ATAGTTTAGCGGCCGCTTATAACCAAGAACCAGCAATA | |