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. 2017 May 31;83(12):e00654-17. doi: 10.1128/AEM.00654-17

TABLE 2.

Overview of results from extended conjugation experiments on agar and in broth

Mating pair (donor→recipient) Mating time (h) Conjugation result at/ina:
25°C
 30/37°Cb
Agar Brothc Agar Brothc
E. coli 1292(IncK)→E. coli 6927-5 4 NA NA + +
24 + + + +
48 + + + +
E. coli 2798(IncI1)→E. coli 6927-5 4 NA NA + +
24 − + + +
48 − + + +
E. coli 1292(IncK)→S. marcescens 3306 4 NA NA − +
24 + − − +
48 − − − +
E. coli 1292(IncK)→S. marcescens 3307 4 NA NA + +
24 + + + +
48 + + + +
S. marcescens 3306 transconjugant (IncK)→E. coli 6927-5 4 NA NA + +
24 + + + +
48 + + + +
S. marcescens 3307 transconjugant (IncK)→E. coli 6927-5 4 NA NA + +
24 + + + +
48 + + + +
S. marcescens 3307 transconjugant (IncK)→S. proteamaculans 5685 4 NA NA + +
24 − − + +
48 − + + +
a

+, confirmed transfer of plasmid carrying blaCMY-2; −, no observed transfer of blaCMY-2-carrying plasmid; NA, not applicable.

b

30°C was applied for all matings involving Serratia spp., while 37°C was applied for matings involving E. coli only.

c

Transconjugant detection limit, 10 CFU/ml.