FIG 2.

Specific binding of DyLight488-labeled Cry8-like to BBMVs from H. oblita. A fixed amount of BBMV proteins (2 μg) was incubated with increasing concentrations (from 0 to 80 nM) of DyLight488-labeled Cry8-like. Nonspecific binding was analyzed in the presence of a 100-fold molar excess of unlabeled homologous protein. Nonspecific binding was subtracted from total binding, resulting in specific binding, which is shown. Each data point represents the mean calculated from at least two independent experiments. Error bars represent standard errors.