FIG 3.

Homologous and heterologous competition binding experiments analyzing the binding of DyLight488-labeled Cry8-like to BBMVs from H. oblita. Curves represents the total binding of DyLight488-labeled Cry8-like (20 nM) to BBMV proteins (2 μg) with increasing amounts of unlabeled Cry8-like or Cry8Ga proteins as competitors, as indicated. After binding interactions, the proteins were analyzed by 10% SDS-PAGE and the gels were scanned for green fluorescence (excitation at 488 nm and emission at 518 nm) in a Typhoon Trio scanner. Densitometric analysis of the fluorescence signals collected from the gels was conducted with ImageJ software, to quantify binding. Each data point depicts the mean calculated from at least two independent experiments. Error bars depict standard errors.