FIG 6.

SDS-PAGE of midgut BBMV proteins from H. oblita. Lane 1, whole solubilized BBMV proteins from H. oblita; lane 2, H. oblita BBMV proteins bound to Cry8-like (the arrowheads indicate the protein bands bound to Cry8-like that were cut out and analyzed by LC-MS/MS); lane 3, Cry8-like-Sepharose beads without incubation with solubilized BBMV proteins; lane 4, BBMV proteins bound to beads without coupled Cry8-like; lane 5, unbound BBMV proteins after incubation with negative-control beads. The numbers at the left indicate the molecular sizes (M) of protein markers, in kilodaltons. The gel was silver stained.