TABLE 1.

Hydrolysis kinetic parameters of LaPul13_14 toward oligomeric and polymeric α-1,6-branched glucans at 37°C and pH 5.0

Substrate	Km (mg ml−1)	kcat (s−1)	kcat Km−1 (ml s−1 mg−1)	Normalized kcat Km−1
Pullulan	0.05 ± 0.004	518 ± 10.5	10,368	100
Amylopectin	0.37 ± 0.041	25 ± 0.7	67	0.6
β-Limit dextrin	0.20 ± 0.090	189 ± 15.8	945	9
62-α-d-Maltosyl maltotriose	0.33 ± 0.040a	378 ± 18.0	1,145b

^aMillimolar.

^bPer millimolar per second.