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. 2017 Mar 31;13(6):4238–4244. doi: 10.3892/ol.2017.5962

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Copyright: © Cai et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Association of Akt1 with p300 in IL-17A-treated nasopharyngeal carcinoma cells. C666-1 cells were stimulated with 10 ng/ml IL-17A for 30 min. Western blot analysis revealed that the expression of p300, CBP and PCAF was not induced by IL-17A stimulation. Further co-immunoprecipitation analysis demonstrated that the interaction of Akt1 with p300, but not with CBP or PCAF, was induced in C666-1 cells in response to IL-17A stimulation for 30 min. The data are from one experiment (n=3 in each group), and are representative of three independent experiments. Representative western blot photographs were produced. IL-17A, interleukin-17A; IgG, immunoglobulin G; CBP, CREB-binding protein; p300, E1A binding protein p300; PCAF, p300/CBP-associated factor; Akt1, AKT serine/threonine kinase 1; iso, isotype; IP, immunoprecipitation.