Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Mar 31;13(6):4238–4244. doi: 10.3892/ol.2017.5962

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Cai et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 4. — Role of p300 in Akt1 acetylation and phosphorylation in nasopharyngeal carcinoma cells following exposure to IL-17A. C666-1 cells were transfected with p300 shRNA or control shRNA for 48 h prior to IL-17A stimulation. Co-immunoprecipitation and western blot analysis were further used to detect Akt1 acetylation and phosphorylation at 30 min following IL-17A stimulation. The results showed that p300 shRNA suppressed Akt1 acetylation and phosphorylation in C666-1 cells following exposure to IL-17A compared to the control shRNA treatment. **P<0.01 vs. control shRNA+IL-17A group. The data are from one experiment, and are representative of three independent experiments. Results are presented as the mean ± standard deviation (n=3 in each group). Representative western blot photographs were produced. IL-17A, interleukin-17A; IgG, immunoglobulin G; Ack, acelytated; Akt1, AKT serine/threonine kinase 1; p, phosphorylated; t, total; iso, isotype; IP, immunoprecipitation; p300, E1A binding protein p300; sh, short hairpin.