Figure 6.

SIRP-α decreases COX-2 expression and cytokine production by negatively regulating p38 MAPK and NF-κB. (A) Cells were transfected with VT, KD and OV, together with the NF-κB or AP-1 reporter plasmids (0.2 µg), and the control plasmid pRL-TK (0.02 µg), and then luciferase activities were detected. (B) Cells were transfected with VT, KD, together with various key protein inhibitors, and then the expression of COX-2 were determined. SIRP, signal regulatory protein; COX-2, cyclooxygenase-2; MAPK, mitogen activated protein kinase; NF, nuclear factor; VT, control vector; KD, shRNA for SIRP-α; OV, pcDNA3.1-SIRP-α; AP-, activator protein-; pRL-TK, thymidine kinase promoter-Renilla luciferase reporter plasmid.