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. 2017 Apr 5;13(6):4231–4237. doi: 10.3892/ol.2017.5992

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Copyright: © Wu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Effects of Wnt10B knockdown on the inhibition of gastric cancer cell proliferation and migration. (A) SGC-7901 cells were infected with lentivirus carrying Wnt10B shRNA or negative control shRNA and expression of Wnt10B mRNA was analyzed by RT-qPCR. (B) Duplicated cells were subjected to western blot analysis of Wnt10B protein expression. (C) Cell counting assay. Growth curves of Wnt10B-knockdown SGC-7901 cells (shWnt10B) and control cells (shGFP) are presented as the mean ± standard deviation and plotted in the graph. (D) Immunofluorescence analyses of Ki67 expression in Wnt10B knocked down SGC-7901 (shWnt10B) and control cells (shGFP). Original magnification, ×400. (E) Western blot analyses of Ki67 expression in Wnt10B-knockdown SGC-7901 (shWnt10B) and control cells (shGFP). (F) Wound healing assay and (G) Transwell migration assay. SGC-7901 cells were infected with lentivirus carrying Wnt10B shRNA or negative control shRNA and subjected to wound healing and Transwell migration assays. Original magnification, ×100. **P<0.01 and ***P<0.001, compared with control cells. GFP, green fluorescent protein; shRNA, short hairpin RNA; RT-qPCR, reverse transcription polymerase chain reaction.