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. 2017 Apr 5;13(6):4231–4237. doi: 10.3892/ol.2017.5992

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Copyright: © Wu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effects of Wnt10B knockdown on the regulation of gene expression. (A) Western blot analysis of β-catenin, cyclin D1, E-cadherin and N-cadherin expression in Wnt10B knocked down SGC-7901 (shWnt10B) and negative control cells (shGFP). (B) Immunofluorescence analyses of E-cadherin and N-cadherin expression in Wnt10B knocked down SGC-7901 (shWnt10B) and negative control cells (shGFP). Original magnification, ×400. (C) Western blot analysis of stem cell markers Oct4 and Nanog expression in Wnt10B knocked down SGC-7901 (shWnt10B) and negative control cells (shGFP). (D) Correlation between Wnt10B and Oct4 and Nanog mRNA levels in gastric cancer tissues. There was a positive correlation between mRNA levels of expression. Oct4, octamer-binding transcription factor 4; shRNA, short hairpin RNA; GFP, green fluorescent protein.