Table 1. Cytotoxic activities of cucurbitacins and reference drugs against Hela, MCF-7 and U2OS cells.

Compounds	IC80			IC50
	Hela	MCF-7	U2OS	Hela	MCF-7	U2OS
Colchicinea	27.01 ± 7.48 nM	79.89 ± 40.85 nM	0.87 ± 1.46μM	14.9 ± 3.94 nM	30.29 ± 8.02 nM	25.2 ± 19.58 nM
Vinblastinea	0.7 ± 0.34μM	2.08 ± 0.92μM	1.15 ± 0.51μM	0.02 ± 0.01 nM	0.06 ± 0.05 nM	0.11 ± 0.07 nM
Latrunculin Bb	63.94 ± 5.68μM	140.1 ±6.58μM	37.17 ± 15.68μM	11.19 ±1.27μM	38.5 ± 1.7μM	5.67 ± 0.59μM
Cucurbitacin B	22 ± 1.39 nM	43.71 ± 10.61 nM	28.05 ± 15.25 nM	12.2 ± 1.42 nM	22.93 ± 4.51 nM	17.07 ± 4.55 nM
Cucurbitacin E	15.09 ± 2.67 nM	0.92 ± 0.2μM	26.27 ± 18.50 nM	6.43 ± 1.05 nM	54 ± 3.16 nM	15.07 ± 4.51 nM
Cucurbitacin I	55.49 ± 3.63 nM	0.29 ± 0.08μM	34.03 ± 17.74 nM	44.77 ± 1.54 nM	64.67 ± 14.29 nM	23.47 ± 16.92 nM

Notes.

^aActive on tubulin/microtubules.

^bActive against actin filaments; data are presented as mean ± SD.