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. 2017 Apr 20;13(6):4727–4733. doi: 10.3892/ol.2017.6052

Table I.

Sequences of miR-21 inhibitor and control oligonucleotides, and primers used in their construction.

Oligonucleotide Sequence
miR-21 inhibitor 5′-TCAACATCAGTCTGATAAGCTA-3′
  Forward, BamHI 5′-GATCCTCAACATCAGTCTGATAAGCTACGATTCAACATCAGTCTGATAAGCTAACCGGTTCAACATCAGTCTGATAAGCTATCACTCAACATCAGTCTGATAAGCTATTTTTTGAATT-3′
  Reverse, EcoRI 5′-ACCGGTTAGCTTATCAGACTGATGTTGAATCGTAGCTTATCAGACTGATGTTGAGAATTCAAAAAATAGCTTATCAGACTGATGTTGAGTGATAGCTTATCAGACTGATGTTGA-3′
miR-NC 5′-TTCTCCGAACGTGTCACGT-3′
  Forward, BamHI 5′-GATCCGTTCTCCGAACGTGTCACGTTTCAAGAGAACGTGACACGTTCGGAGAACTTTTTTG-3′
  Reverse, EcoRI 5′-AATTCAAAAAAGTTCTCCGAACGTGTCACGTTCTCTTGAAACGTGACACGTTCGGAGAACG-3′

miR, microRNA; NC, negative control.