Table I.
Oligonucleotide | Sequence |
---|---|
miR-21 inhibitor | 5′-TCAACATCAGTCTGATAAGCTA-3′ |
Forward, BamHI | 5′-GATCCTCAACATCAGTCTGATAAGCTACGATTCAACATCAGTCTGATAAGCTAACCGGTTCAACATCAGTCTGATAAGCTATCACTCAACATCAGTCTGATAAGCTATTTTTTGAATT-3′ |
Reverse, EcoRI | 5′-ACCGGTTAGCTTATCAGACTGATGTTGAATCGTAGCTTATCAGACTGATGTTGAGAATTCAAAAAATAGCTTATCAGACTGATGTTGAGTGATAGCTTATCAGACTGATGTTGA-3′ |
miR-NC | 5′-TTCTCCGAACGTGTCACGT-3′ |
Forward, BamHI | 5′-GATCCGTTCTCCGAACGTGTCACGTTTCAAGAGAACGTGACACGTTCGGAGAACTTTTTTG-3′ |
Reverse, EcoRI | 5′-AATTCAAAAAAGTTCTCCGAACGTGTCACGTTCTCTTGAAACGTGACACGTTCGGAGAACG-3′ |
miR, microRNA; NC, negative control.