Skip to main content
. Author manuscript; available in PMC: 2018 Feb 15.
Published in final edited form as: J Am Chem Soc. 2017 Feb 3;139(6):2351–2358. doi: 10.1021/jacs.6b11737

Figure 1. Expanding the bioluminescence toolkit with unique enzyme-substrate pairs.

Figure 1

(A) Luciferase-mediated light production proceeds via an adenylation–oxidation sequence. (B) Strategy to develop orthogonal luciferase-luciferin pairs via substrate resolution. Genetically engineered luciferases and chemically modified luciferins were screened to identify novel partners. Only complementary enzyme-substrate pairs interact to produce light. (C) Model of D-luciferin bound to firefly luciferase (Fluc). (D) Synthesis of C7′ (left) and C4′ (right) sterically modified luciferins.