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. Author manuscript; available in PMC: 2018 Feb 15.
Published in final edited form as: J Am Chem Soc. 2017 Feb 3;139(6):2351–2358. doi: 10.1021/jacs.6b11737

Figure 5. Imaging cells with orthogonal luciferase-luciferin pairs.

Figure 5

(A) Mutant luciferase-expressing DB7 cells were plated (1.5 x 105 cells/well) in 96-well black plates and sequentially incubated with C4′ and C7′ sterically modified luciferins (750 μM). Representative bioluminescence images are shown. (B) Quantification of the images from (A) after initial substrate addition. Error bars represent the standard error of the mean for experiments performed in triplicate.