Figure 2.

miR-375 suppresses cell growth and induces cell cycle arrest in EC109 cells. (A) Reverse transcription-quantitative polymerase chain reaction analysis of miR-375 expression in miR-375 mimic- or NC-transfected EC109 cells. (B) The growth rate of miR-375 mimic- or NC-transfected EC109 cells was measured using a Cell-Counting kit 8 assay. (C) Flow cytometric analysis of the cell cycle distribution of miR-375 mimic- or NC-transfected EC109 cells. (D) Flow cytometric analysis of apoptosis revealed no difference in apoptosis rates between miR-375 mimic- and NC-transfected EC109 cells. Experiments were performed in triplicate and data are expressed as the mean ± standard deviation. *P<0.05 vs. NC. miR-375, microRNA-375; NC, negative control; OD, optical density; PI, propidium iodide; FITC, fluorescein isothiocyanate.