Table I.

Primers.

Gene	Experiment	Type	Sequence (5′-3′)	Product size	Annealing temperature
RFX1	RT-qPCR	Forward	GCAGACAGAAGTGGGGAGAA	241 bp	60°C
		Reverse	CAGTATACGCCTGTGTTGCC
	Bisulfite sequencing in promoter region	Forward	TGTTTTTGAGGGTTTAGTTTTTTTT	207 bp	56°C
		Reverse	ACAACTATTACTACCCACCCTAATTAC
	Bisulfite sequencing in intron 7	Forward	GGTGGAGGTTTGGAGTTT	273 bp	60°C
		Reverse	ACAAAAACAAATATAAAAACAACA
	MSP	Forward	TTTTTCGTTTTTATTTAATTTCGAC	167 bp	56°C
		Reverse	ATTTCTAACTTCTTACGCTAACGTC
	U-MSP	Forward	GGAGGTGTTATAGTTATGGTAGTTGT	164 bp	56°C
		Reverse	CTTTTCAAAAATCTCAAAATCAAT
GAPDH	RT-qPCR	Forward	GAAGGTGAAGGTCGGAGTC	226 bp	60°C
		Probe	CAAGCTTCCCGTTCTCAGCC
		Reverse	GAAGATGGTGATGGGATTTC

RFX1, regulatory factor X1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; MSP, methylation specific PCR; U-MSP, un-methylation specific PCR.