Table III.
miRNA target filter analysis of differentially expressed target genes and miRNAs from the cell cycle, p53, apoptosis and immune response categories that demonstrated an inverse association in LLC1 cells exposed to single dose (10 Gy) or fractionated dose (5×2 Gy) irradiation.
| 10 Gy | 5×2 Gy | |||
|---|---|---|---|---|
| Category | miRNA | Target gene | miRNA | Target gene |
| Cell cycle | miR-34c-5p↑ | E2f3↓; E2f5↓; Ccne2↓ | miR-30c-5p↑ | Ccne2↓; Stag1↓; Orc4↓; Skp2↓ |
| miR-34c-5p↑ | Ccne2↓; E2f3↓ | |||
| miR-129-5p↑ | Stag1↓; Orc4↓ | |||
| miR-145a-5p↑ | Orc4↓ | |||
| miR-186-5p↑ | Cdc27↓; Stag1↓ | |||
| p53 signaling pathway | miR-34c-5p↑ | Ccne2↓ | miR-30c-5p↑ | Ccne2↓ |
| miR-34c-5p↑ | Ccne2↓ | |||
| miR-129-5p↑ | Pten↓ | |||
| miR-145a-3p↓ | Pmaip1↑; Sesn2↑ | |||
| Apoptosis | miR-30c-5p↑ | Ppp3cb↓ | ||
| Immune response | miR-34b-3p↑ | Spred1↓ | miR-30c-5p↑ | Lepr↓; Kras↓; Ppp3cb↓ |
| miR-34c-3p↑ | Spred1↓ | miR-34c-3p↑ | Gng5↓ | |
| miR-34c-5p↑ | Pdk1↓ | miR-34c-5p↑ | Pdk1↓ | |
| miR-136-5p↓ | Eda2r↑ | miR-129-5p↑ | Il6ra↓; Rock1↓ | |
| miR-145a-3p↓ | Cr2↑; Inpp5d↑ | miR-186-5p↑ | Vegfa↓; Pias2↓ | |
| miR-466a-5p↓ | Eda2r↑; Egfr↑; Inhbb↑ | miR-192-5p↑ | Crk↓; Pias2↓ | |
| miR-710↓ | Stat1↑; Pik3r3↑ | miR-105↓ | Tgfbr2↑; Stat1↑ | |
| miR-145a-3p↓ | Tgfbr2↑; Cr2↑; Inpp5d↑; Ticam1↑ | |||
An upwards and downwards pointing arrow indicates increased and decreased expression, respectively. miRNA/miR, microRNA.