Table IV.
Validation of gene and miRNA microarray data by RT-qPCR.
| A, Gene | ||
|---|---|---|
| Irradiation dose | ||
| Data | 10 Gy | 5×2 Gy |
| Btg2 | ||
| RT-qPCR | 5.09±0.34 | 4.51±0.73 |
| Microarrays | 5.38±1.55 | 2.74±0.09 |
| Ccng1 | ||
| RT-qPCR | 4.02±0.57 | 3.2±0.47 |
| Microarrays | 2.06±0.47 | 2.30±0.45 |
| P21 | ||
| RT-qPCR | 2.64±0.06 | 2.6±0.49 |
| Microarrays | 5.96±1.28 | 2.43±0.16 |
| Thbs2 | ||
| RT-qPCR | 1.30±0.10 | 2.76±0.67 |
| Microarrays | 1.29±0.18 | 2.26±0.11 |
| B, miRNA | ||
| Irradiation dose | ||
| Data | 10 Gy | 5×2 Gy |
| miR-34b-3p | ||
| RT-qPCR | 2.76±0.48 | 3.6±0.58 |
| Microarrays | 4.07±1.53 | 14.78±4.75 |
| miR-34c-5p | ||
| RT-qPCR | 2.67±0.66 | 2.32±0.34 |
| Microarrays | 2.79±0.33 | 5.3±0.90 |
| miR-186-5p | ||
| RT-qPCR | 1.62±0.48 | 2.28±0.06 |
| Microarrays | 1.55±0.59 | 3.55±0.81 |
| miR-145a-5p | ||
| RT-qPCR | 1.41±0.46 | 3.44±0.49 |
| Microarrays | 1.12±0.88 | 6.99±1.41 |
miRNA/miR, microRNA; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.