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. 2017 Apr 3;13(6):4526–4532. doi: 10.3892/ol.2017.5971

Figure 6.

Figure 6.

Overexpression of TIMP1 reduces migration of cervical cancer cells and downregulates MMP9. (A) TIMP1 mRNA levels were significantly upregulated in HeLa and C33A cells following transfection with MigRI-TIMP1-GFP relative to transfection with empty vector. (B) TIMP1 protein levels were markedly upregulated in HeLa and C33A cells following transfection with MigRI-TIMP1-GFP compared with transfection with empty vector. β-actin was used as a loading control. (C) Overexpression of TIMP1 markedly inhibited migration in cervical cancer cells compared with transfection with empty vector. (D) Quantification of migrating cells in HeLa and C33A cells. Overexpression of TIMP1 significantly decreased the number of migrating cells compared with transfection with empty vector. (E) Expression of MMP9 mRNA was significantly downregulated in HeLa and C33A cells following overexpression of TIMP1 compared with transfection with empty vector. (F) Expression of MMP9 protein was markedly downregulated in HeLa and C33A cells following overexpression of TIMP1 compared with transfection with empty vector. β-actin was used as a loading control. *P<0.05 vs. transfection with empty vector. TIMP1, tissue inhibitor of metalloproteinase 1; MMP9, matrix metalloproteinase 9; GFP, green fluorescent protein.