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. 2017 Apr 5;13(6):4224–4230. doi: 10.3892/ol.2017.5994

Figure 3.

Figure 3.

Induction of CTSB by CXCL9 and CXCL10 in the human breast cancer cell lines MCF-7 and MDA-MB-231. (A) These cell lines express splice variants CXCR3-A and CXCR-B as demonstrated by western blot analysis using antibody MAB160. (B) Flow cytometric detection of CXCR3 protein expression on the cell surface of living cell lines, employing antibody MAB160 (shaded curves). Solid lines, isotype control antibody. (C) Western blot analysis demonstrating induction of cellular pro-CTSB and CTSB by CXCL9 or CXCL10 in MCF-7 and MDA-MB-231 cells, using antibody MAB965. (D) Detection of CTSB in supernatants of MDA-MB-231 cells upon stimulation with CXCL9 and CXCL10, but not with CXCL4. ***P≤0.001 CTSB, cathepsin B; CXCL, C-X-C motif chemokine ligand; CXCR3, C-X-C motif chemokine receptor.