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Bcl-2 serves a role in SFN/paclitaxel-induced apoptosis. (A) Western blot analysis of Bcl-2 protein expression in MDA-MB-231 cells treated with 5 µM SFN alone, 10 nM paclitaxel alone, or 10 nM paclitaxel and 5 µM SFN for 24 h. (B) MDA-MB-231 cells transfected with Bcl-2 or the vector control were analyzed for activated Cas-3 using western blot analysis. (C) Flow cytometric analysis of positive Annexin V staining. *P<0.05; ***P<0.001. SFN, sulforaphane; Cas, caspase.
