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. 2016 Jul 19;101(6):1054–1068. doi: 10.1111/mmi.13441

Figure 6.

Figure 6

LmRad9 and LmHus1 deficiency has distinct effects on DNA damage persistence upon HU and phleo treatment.

A. WT, LmHus1+/− and LmRad9+/− cells were incubated with 5mM HU for ∼10 h; after washing, cells were incubated in HU‐free medium and collected at the indicated time points; Pre indicate extracts prepared right before HU addition; extracts prepared in each time point were analyzed by western blot with anti‐γH2A antibody; EF1α was used as a loading control; graph at right shows quantification or γH2A signal as normalized with EF1α signal; vertical lines on top of each bar indicate standard deviation.

B. WT, LmHus1+/− and LmRad9+/− cells were incubated with 5 μg/ml of phleo for the indicated periods of time; extracts prepared in each time point were analyzed by western blot with anti‐γH2A antibody; EF1α was used as a loading control; graph at right shows quantification or γH2A signal as normalized with EF1α signal; vertical lines on top of each bar indicate standard deviation.