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Comparison of biochemical characteristics of different HRP preparations. Kinetic constants were determined for ABTS/H2O2.
| HRP preparation | Km [mM] | vmax [U mg−1] | vmax Km−1 [U mg−1 mM−1] | τ1/2 at 60 °C [min] |
|---|---|---|---|---|
| plant HRP | 1.75 ± 0.12 | 567 ± 39 | 324 | 53 |
| soluble HRP P. pastoris | 1.50 ± 0.04 | 152 ± 5 | 101 | 34 |
| refolded HRP E. coli | 1.25 ± 0.10 | 62.5 ± 5.0 | 50 | 25 |
| soluble HRP E. coli | 0.83 ± 0.21 | 12.7 ± 0.05 | 15 | 1.1 |
