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. 2014 Feb 25;7(3):1455–1472. doi: 10.3390/ma7031455

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© 2014 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 4. — (A) Scheme of procedure of isolation using magnetic particles (MPs)—1 mixture of adenine nucleotides with MPs, 2 immobilization of adenine nucleotides to MPs, 3 removing contaminants, 4 washing MPs coupled adenine nucleotide, 5 removing contaminants, 6 elution of adenine oligonucleotides from MPs, 7 removing adenine oligonucleotides, 8 purification; (B) Dependence of concentration of isolated adenine nucleotides related to the applied concentration of adenine nucleotides. The calculated quadratic dependences are based on the data measured for peak A within the concentration range from 50 ng/mL to 25 μg/mL of adenine nucleotides before isolation process; (C) Determined concentration of adenine nucleotides related to the local maxima/minima of calculated dependences for peak A after isolation process; not restricted to model concentration interval of adenine nucleotides; (D) Dependence of quadratic coefficient of calculated quadratic dependences on various number of adenine bases in strand (related to part B); (E) Dependence of linear coefficient of calculated quadratic dependences on various number of adenine bases in strand (related to part B).