. Author manuscript; available in PMC: 2018 Jan 10.

Published in final edited form as: Biochemistry. 2016 Dec 27;56(1):33–46. doi: 10.1021/acs.biochem.6b00943

Table 4.

IC₅₀ Values for NVP with WT HIV-1 and HIV-1 K103N RT at 0.25 and 6 mM Mg²⁺

	condition^a	IC₅₀^b (μM)	x-fold decrease^c (p value)
HIV-1 RT	6 mM Mg²⁺	16 ± 0.5	–
	0.25 mM Mg²⁺	4.3 ± 0.7	3.7 (<0.00001)
K103N RT	6 mM Mg²⁺	49 ± 5	–
	0.25 mM Mg²⁺	14 ± 3	3.5 (0.0003)

Assays were as described in Experimental Procedures. The single template-directed nucleotide used in this assay was a dATP opposite T (5′ T template). All assays used 25 nM RT.

Half-maximal inhibitory values (IC₅₀) were calculated, as described in Experimental Procedures, from the relationship between the concentration of nevirapine and the percent of inhibition achieved at the particular inhibitor concentration. Results are averages of three experiments ± SD.

The x-fold decrease in IC₅₀ values compared to the 6 mM result (number above) with the same RT. A lower IC₅₀ value at 0.25 mM Mg²⁺ confirms better inhibition with the lower Mg²⁺ concentration. Values were calculated using a standard Student’s t test.